Evaluation Of Immunomodulatory Activity Of Ketorolac In Mice
By: Mahtab Anwer (2013-VA-846)
| Dr. Aqeel Javeed.
Contributor(s): Dr. Muhammad Ovais Omer | Dr. Aamir Ghafoor.
Material type: 
BookPublisher: 2015Description: 65p.Subject(s): Department of Pharmacology & ToxicologyDDC classification: 2441-T Dissertation note: Ketorolac is one of the non-steroidal anti-inflammatory drugs (NSAIDs) commonly prescribed to treat postoperative pain and reduced stress response.The present study was designed to evaluate the immunomodulatory activity of ketorolac.In each assay, 25 mice were used. All the mice were divided randomly into 5 groups. Each group had 5 mice. Negative control group was treated with solvent, positive control group was treated with cyclophosphamide and other three groups were injected intraperitoneally at the three different doses of ketorolac (2mg/kg, 4mg/kg and 8mg/kg). Delayed type hypersensitivity assay (DTH) and cyclophosphamide induced neutropenia assays were performed to evaluate the cell-mediated immune activity of ketorolac. While, the effect of ketorolac on humoral immunity was determined by performing heamagglutination assay and mice lethality test.It was observed that significant reduction in skin thickness and white blood cells and neutropenia in dose dependent manner of ketorolac treated groups (8mg/kg ketorolac > 4mg/kg ketorolac > 2mg/kg ketorolac).Significant reduction in HAtiter values in dose dependent manner of ketorolac treated groups were also evaluated (8mg/kg ketorolac >4mg/kg ketorolac >2mg/kg ketorolac).In mice lethality assay, mortality ratio was maximum in 8mg/kg ketorolac treated group which was 100%. In 4mg/kg ketorolac group and positive control group showed the 80% mortality and 2mg/kg ketorolac treated group showed the 40% mortality. Minimum mortality was observed in negative control group. From these results, ketorolac exhibited the immunosuppressive effect. This study may have potential impacts of ketorolac in clinical applications besides its analgesic and anti -inflammatory properties.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 2441-T (Browse shelf) | Available | 2441-T |
Ketorolac is one of the non-steroidal anti-inflammatory drugs (NSAIDs) commonly prescribed to treat postoperative pain and reduced stress response.The present study was designed to evaluate the immunomodulatory activity of ketorolac.In each assay, 25 mice were used. All the mice were divided randomly into 5 groups. Each group had 5 mice. Negative control group was treated with solvent, positive control group was treated with cyclophosphamide and other three groups were injected intraperitoneally at the three different doses of ketorolac (2mg/kg, 4mg/kg and 8mg/kg). Delayed type hypersensitivity assay (DTH) and cyclophosphamide induced neutropenia assays were performed to evaluate the cell-mediated immune activity of ketorolac. While, the effect of ketorolac on humoral immunity was determined by performing heamagglutination assay and mice lethality test.It was observed that significant reduction in skin thickness and white blood cells and neutropenia in dose dependent manner of ketorolac treated groups (8mg/kg ketorolac > 4mg/kg ketorolac > 2mg/kg ketorolac).Significant reduction in HAtiter values in dose dependent manner of ketorolac treated groups were also evaluated (8mg/kg ketorolac >4mg/kg ketorolac >2mg/kg ketorolac).In mice lethality assay, mortality ratio was maximum in 8mg/kg ketorolac treated group which was 100%. In 4mg/kg ketorolac group and positive control group showed the 80% mortality and 2mg/kg ketorolac treated group showed the 40% mortality. Minimum mortality was observed in negative control group. From these results, ketorolac exhibited the immunosuppressive effect. This study may have potential impacts of ketorolac in clinical applications besides its analgesic and anti -inflammatory properties.
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